Blocking Buffer (1% BSA in 1XPBS) is made of high-quality BSA that is useful for saturating excess protein-binding sites on membranes and microplates in immunoassays. A good blocking buffer for a specific experiment will bind to all potential sites of nonspecific interaction, eliminating background without altering or obscuring the epitope for antibody binding. Blocking Buffer (1% BSA in 1X PBS) provides a standard concentration of protein for the reduction of background interference in antibody based assays. It facilitates surface saturation during molecular protein research testing and routine ELISA workflows using phosphate buffered saline.
Blocking buffer (1% BSA in 1X PBS) is made of high-quality BSA that is useful for saturating excess protein-binding sites on membranes and microplates in immunoassays. A good blocking buffer for a specific experiment will bind to all potential sites of nonspecific interaction, eliminating background without altering or obscuring the epitope for antibody binding. This Blocking Buffer is a ready-to-use solution which can be used for blocking steps in Western blot, ELISA, IHC and nucleic acid detection methods.