Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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5X Laemmli Buffer
SKU:
ML121
Availability: In stock
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$5.00
5X Laemmli Buffer is specifically used for protein sample preparation prior to loading on SDSPAGE. It is added to each protein sample at 1:4 ratio and boiled for 1-5 min. The use of Laemmli sample buffer ensures optimal band resolution when preparing proteins for SDSPAGE with Tris-glycine-SDS running buffer. 5X Laemmli Buffer is a concentrated denaturing solution that facilitates the binding of SDS to polypeptide chains. It ensures complete protein denaturation for standardized SDS PAGE analysis and molecular protein research testing of clinical samples.
5X Laemmli Buffer is used as a protein sample loading buffer for use in SDS-PAGE and Western Blotting. This buffer contains bromophenol blue (BPB), SDS, glycerol and Tris-HCl. It is added to each protein sample at 1:4 ratio and boiled for 1-5 min. The use of Laemmli sample buffer ensures optimal band resolution when preparing proteins for SDS-PAGE with Tris-glycine-SDS running buffer. A reducing agent like DL-DTT or 2-Mercaptoethanol can be added prior to sample preparation.
| Product Name | 5X Laemmli Buffer |
|---|---|
| SKU | ML121 |
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