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5X Laemmli Buffer is used as a protein sample loading buffer for use in SDS-PAGE and Western Blotting. This buffer contains bromophenol blue (BPB), SDS, glycerol and Tris-HCl. It is added to each protein sample at 1:4 ratio and boiled for 1-5 min. The use of Laemmli sample buffer ensures optimal band resolution when preparing proteins for SDS-PAGE with Tris-glycine-SDS running buffer. A reducing agent like DL-DTT or 2-Mercaptoethanol can be added prior to sample preparation.
| Product Name | 5X Laemmli Buffer |
|---|---|
| SKU | ML121 |
| Customized Product Available | No |
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