10X Tris acetate-SDS Running Buffer is used for Tris-Acetate Gels during protein electrophoresis.
It resolves native proteins more effectively than the Tris-Glycine gel system. This buffer contains 500mM Tris base, 500mM Tricine, 3.5mM SDS mixed in a ratio to achieve pH 8.23. No acid or base is added to the buffer for this pH adjustment. Mainly used for high molecular weight proteins.