5X Protein Loading Buffer (Reducing) can be used as a loading buffer in all kinds of denaturing gels and are compatible with coomassie and silver staining and Western blotting. The marker dye transfers and binds only to nitrocellulose. It contains premixed reducing agent for disruption of protein structures
5X Protein Loading Buffer (Reducing) is a unique loading buffer due to the presence of a pink tracking dye which runs as the dye front during SDS-PAGE and transfers completely to the nitrocellulose membrane while performing Western blotting protocol with no interference, helping to determine the efficiency of transfer as well as to align the cut membrane. This loading buffer contains SDS and DTT which are required for complete disruption of all higher order protein structures and glycerol for proper loading of the protein sample.