10X TBST (Tris buffered saline with Tween-20®) solution is extensively used in several molecular biological and immunological experiments especially as a wash buffer in western blotting and ELISA.
10X TBST is Tris buffered saline (TBS) solution with the detergent Tween-20® and is mainly used as a wash buffer for immunohistochemical staining and in situ hybridization procedures as it reduces nonspecific background staining. It contains 100 mM Tris, 1.5 M Sodium chloride and 0.5% Tween 20®. It is commonly used as a wash solution for western blot membranes and microtiter plate wells in ELISA assays.