Blocking Buffer

Blocking buffer is used for the reduction of background interference during Western blotting, ELISA, immunostaining or other immunoassays. This buffer prevents non-specific binding of antibodies by binding to any open protein binding sites that do not have antibodies or antigen attached. Blocking Buffer enhances the sensitivity and specificity of any immunochemical applications by increasing signal-to-noise ratio and promoting specific binding of the primary antibody while minimizing non-specific interactions. This buffer comes as a ready-to-use solution of preformulated nonfat dry milk which is suitable for any immunochemical applications. Blocking Buffer provides a stabilized protein solution for the surface saturation of vacant sites on blotting membranes. This reagent facilitates antibody background reduction during molecular protein research testing to improve the signal to noise ratio in immunological assays.