Denaturation Solution denatures the double-stranded DNA and as a result DNA can be efficiently transferred to nylon or nitrocellulose membrane and subsequently hybridized to a labeled probe during neutral Southern blotting procedure. When denaturation solution is used in conjunction with depurination solution, the phosphodiester backbone at the sites of depurination is hydrolysed which allows a very efficient transfer of large DNA to the membrane. Denaturation Solution is a concentrated caustic reagent used to convert double stranded nucleic acids into single strands within a gel matrix. This Denaturation Solution provides the necessary Alkaline Treatment for Strand Separation during any professional Molecular Hybridization Prep and genetic analysis.
Denaturation Solution denatures the double-stranded DNA and as a result DNA can be efficiently transferred to nylon or nitrocellulose membrane and subsequently hybridized to a labeled probe during neutral Southern blotting procedure. When denaturation solution is used in conjunction with depurination solution, the phosphodiester backbone at the sites of depurination is hydrolysed which allows a very efficient transfer of large DNA to the membrane. It is composed of Sodium chloride and strong base Sodium hydroxide