Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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HiTouch™ SCDA w/ Polysorbate 80 & Lecithin Flexi Plate™ (γ-irradiated)
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$185.90
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SKU:
FL035G
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HiTouch™ Flexi Plates#4fa25a
Intended Use
For determining the efficiency of sanitization of containers, equipment surfaces, water miscible cosmetics, etc. It can also be used to enumerate the organisms from water insoluble products and fatty products containing preservatives or antimicrobials.
Composition**
| Ingredients | Gms / Litre |
|---|---|
| Casein enzymic hydrolystae | 15.000 |
| Papaic digest of soyabean meal | 5.000 |
| Sodium chloride | 5.000 |
| Lecithin | 0.700 |
| Polysorbate 80 (Tween 80) | 5.000 |
| Agar | 15.000 |
**Formula adjusted, standardized to suit performance parameters
Directions
Open the pouch in the protected area. Remove the wrappping and open the lid and carefully lift up the enclosed prepared medium plate so as to avoid touching the agar surface by hand. Touch the surface of agar plate onto the surface to be tested. Gently press the plate manually for upto 10 sencond. Apply constant and uniform pressure to the whole surface (ensuring that an even pressure of 25 gm/cm2 is distributed over the whole plate for 10 seconds). Replace exposed medium plate back in the base plate. Close the lid. Press the sides of the lid to make sure that it is fixed in the grooves. Disinfect the surface where the sample was taken in order to remove any possible traces of agar. Incubate the plates at specified temperature. After incubation as recommended count the number of colonies which have appeared on the surface of medium. Alternative Methods of Inoculation : To use as Culture Plate (ii), Sample Dilution Plate (iii) or Swabbing Plate (iv) To use as Gravitation Settling Plate (v)
Principle And Interpretation
Hitouch Flexi Plates are specially developed for the microbial testing in food, pharmaceutical, cosmetic, dairy, hospitals, water works, environmental testing etc. These plates are handy and ready to use sterile media supplied in flexible disposable plates, 55 mm in diameter. It is grid scored on the base and is irradiated to ensure perfect sterility. Medium is filled aseptically and each plate is packed in pre-sterilized plastic bag. Hitouch Flexi Plateis then packed in plastic pouch wrapping. The unique flexible plate configuration ensures close contact even with uneven surfaces. where not only counts are obtained but it is also possible to select and differentiate between groups of microorganisms like coliforms (both E. coli and non E. coli ). These plates are specially developed for microbial testing, The Flexi plate medium formula is suitable for enumeration of the organisms from water soluble products, fatty products containing preservatives or antimicrobials and the grids enable direct reading on the plates of the number of colonies per cm2. This plate can be used for determining the effeciency of sanitization of containers, equipment surfaces, water miscible cosmetic, etc.
Casein enzymic hydrolysate and papaic digest of soyabean meal provide nitrogenous compounds and other nutrients essential for microbial replication. Lecithin and polysorbate 80 (Tween 80) are neutralizers reported to inactivate residual disinfectants from where the sample is collected (4). Lecithin neutralizes quaternary ammonium compounds and polysorbate 80 neutralizes phenolic disinfectants, hexachlorophene, formalin and with lecithin ethanol (5).
Collection of samples from areas before and after the treatment with disinfectant evaluates cleaning procedures in environmental sanitation. The presence and number of microorganisms is determined by the appearance of colonies on the agar surface (6). After counting the colonies, carry out biochemical testing for identification.
Quality Control
Appearance
Sterile plastic plate containing light to medium amber coloured firm gel.
Quantity of Medium
9 ml of gel in plastic plate.
Reaction
7.10-7.50
Sterility test
Passes release criteria
Cultural response
Cultural characteristics observed after incubation at 35-37°C for 18-24 hours.
| Organism | Growth |
|---|---|
| Escherichia coli ATCC 25922 | Luxuriant |
| Staphylococcus aureus ATCC 25923 | Luxuriant |
| Staphylococcus aureus ATCC 6538 | Luxuriant |
| Pseudomonas aeruginosa ATCC 27853 | Luxuriant (may have green pigment) |
Storage and Shelf Life
Store between 2-8°C. Use before expiry date on the label.
| Product Name | HiTouch™ SCDA w/ Polysorbate 80 & Lecithin Flexi Plate™ (γ-irradiated) |
|---|---|
| SKU | FL035G |
| Customized Product Available | No |
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