Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Sabouraud Dextrose Agar Medium 4, Granulated
As low as
$157.30
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Availability: In stock
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SKU:
GMM063
Recommended for cultivation of yeasts, moulds and aciduric microorganisms in accordance with Indian Pharmacopoeia 2014, Granulated SDA, SD Agar
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Granulated™ Media#4fa25a
Intended Use
Sabouraud Dextrose Agar Medium 4, granulated is used for the cultivation of yeasts, moulds and aciduric bacteria in accordance with Indian Pharmacopoeia, 2014.
Composition**
| Ingredients | Gms/Litre |
|---|---|
| Peptones (meat and casein) | 10.000 |
| Dextrose monohydrate | 40.000 |
| Agar | 15.000 |
| Final pH (at 25°C) | 5.6±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 61.36 grams (the equivalent weight of dehydrated medium per litre) in 1000 ml purified/ distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes or as per validated cycle. Cool to 45-50°C. Mix well and pour into sterile Petri plates.
Principle And Interpretation
Sabouraud Dextrose Agar is Carliers modification (1) of the formulation described by Sabouraud (2) for the cultivation of fungi (yeasts, moulds), particularly useful for the fungi associated with skin infections. The composition of medium is as per I.P (3). This medium is employed for microbial limit tests of food, pharmaceutical, cosmetics, and clinical specimens (4)
Peptones(meat and casein) provides nitrogenous compounds. Dextrose monohydrate provides energy source. High dextrose concentration and low pH favors fungal growth and inhibits contaminating bacteria from clinical specimens (5).
Some pathogenic fungi may produce infective spores, which are easily dispersed in air, so examination should be carried out safety cabinet. For heavily contaminated samples, the plate must be supplemented with inhibitory agents for inhibiting bacterial growth.
Quality Control
Appearance
Cream to yellow coloured granular medium
Gelling
Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium
Light amber coloured clear to slightly opalescent gel forms in Petri plates
Reaction
pH of 6.14% w/v aqueous solution at 25°C (after sterilization). pH : 5.6±0.2
pH
5.40-5.80
Growth Promotion Test
Growth Promotion was carried out in accordance with the method of IP, after an incubation at 30-35 °C for 24-48 hours. Recovery rate is considered as 100% for bacteria growth on Soybean Casein Digest Agar and fungus growth on Sabouraud Dextrose Agar
Growth Promoting Properties
Growth of microorganism comparable to that previously obtained with previously tested and approved lot of medium occurs at the specified temperature for not more than the shortest period of time specified inoculating <= 100 cfu (at 30-35°C for <=24 hours).
Cultural Response
Colonies are comparable in appearance and indication reaction to those previously obtained with previously tested and approved lot of medium occurs for the specified temperature for a period of time within the range specified inoculating <=100cfu (at 30-35°C for 24-48 hours).
| Organism | Inoculum (CFU) | Growth | Recovery | Incubation temperature | Incubation period |
|---|---|---|---|---|---|
| Growth Promotion + Indicative | |||||
| Candida albicans ATCC 10231 | 50-100 | Luxuriant (white colonies) |
>=70% | 30-35 °C | 24-48 hrs |
| Growth Promotion + Total yeast and mould count | |||||
| Candida albicans ATCC 10231 | 50-100 | luxuriant | >=70% | 20-25 °C | <=5 d |
| *Aspergillus brasiliensis ATCC 16404 | 50-100 | luxuriant | >=70% | 20-25 °C | <=5 d |
| Additional Microbiological Testing | |||||
| Candida albicans ATCC 2091 | 50-100 | luxuriant | >=70% | 30-35°C | 24-48 hrs |
| Saccharomyces cerevisiae ATCC 9763 | 50-100 | luxuriant | >=70% | 30-35 °C | 24-48 hrs |
| Escherichia coli ATCC 25922 | 50-100 | good(inhibited on media with low pH) |
>=70% | 30-35 °C | 24-48 hrs |
| Escherichia coli ATCC 8739 | 50-100 | good(inhibited on media with low pH) |
>=70% | 30-35 °C | 24-48 hrs |
| Escherichia coli NCTC 9002 | 50-100 | good(inhibited on media with low pH) |
>=70% | 30-35 °C | 24-48 hrs |
| Trichophyton rubrum ATCC 28191 | good | 20-25 °C | <=7 d | ||
| Lactobacillus casei ATCC 334 | 50-100 | luxuriant | >=70% | 30-35 °C | 24-48 hrs |
Storage and Shelf Life
Store below 30°C and the prepared medium at 2-8°C. Use before expiry date on the label.
| Product Name | Sabouraud Dextrose Agar Medium 4, Granulated |
|---|---|
| SKU | GMM063 |
| Product Type | Granulated |
| Physical Form | Granular |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1.Carlier G. I. M., 1948, Brit. J. Derm. Syph., 60:61. 2.Sabouraud K., 1892, Ann. Dermatol. Syphilol, 3:1061. 3.Indian Pharmocopoeia, 2010, Ministry of Health and Family Welfare, Govt. of India. 4.Bacteriological Analytical Manual, 8th Edition, |
| Customized Product Available | No |
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