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Rappaport Vassiliadis Salmonella Enrichment Broth, Granulated
As low as
$218.90
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Availability: In stock
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SKU:
GMH1491
Recommended for selective enrichment of Salmonella species from pharmaceutical products in accordance with the microbial limit testing by harmonized methodology of USP/EP/BP/JP.
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Granulated™ Media#4fa25a
Intended use
Rappaport Vassiliadis Salmonella Enrichment Broth is recommended for selective enrichment of Salmonella species from pharmaceutical products in accordance with the microbial limit testing by harmonized methodology of USP/EP/BP/JP/IP.
Composition
| Ingredients | g/L |
|---|---|
| Soya peptone | 4.500 |
| Sodium chloride | 8.000 |
| Dipotassium hydrogen phosphate | 0.400 |
| Potassium dihydrogen phosphate | 0.600 |
| Magnesium chloride, hexahydrate | 29.000 |
| Malachite green | 0.036 |
| pH after sterilization (at 25°C) | 5.2±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 27.11 grams of dehydrated medium(the equivalent weight of dehydrated medium per litre) in 1000 ml purified/ distilled water. Heat if necessary to dissolve the medium completely. Dispense as desired into tubes and sterilize by autoclaving at 115°C or as per validated cycle
Principle And Interpretation
Rappaport Vassiliadis Salmonella Enrichment Medium is designed according to the revised formulation by Van Schothorst et al (1) and is recommended for the selective enrichment of Salmonellae from pharmaceutical products. This medium can also be used in direct enrichment of samples containing low inoculum. Present medium is a modification of the Rappaport Vassiliadis Enrichment Broth described by Van Schothorst and Renauld (2). It is prepared in accordance with the harmonized methodology of USP/EP/BP/JP/IP (3-7) has been found to be superior to other Salmonella selective medias. Addition of magnesium chloride to the medium was reported by Peterz et al (8). Salmonella species can be isolated from human faeces without pre-enrichment by using this medium. Salmonella generally survive at little high osmotic pressure, grow at slightly low pH and are resistant to malachite green compared to other bacteria. These characteristics are exploited in this medium for selective enrichment of Salmonella. Magnesium chloride present in the medium raises the osmotic pressure. Natural sugars of soya peptone provide essential growth nutrients and enhance the growth of Salmonella (9). Phosphate buffers the medium to maintain constant pH. Sodium chloride maintains the osmotic balance. Malachite green inhibits many gram-positive bacteria, while selectively enriches Salmonella. The relatively lower concentration of nutrition, also aids selective enrichment of Salmonella. This medium was reported to be superior to Salmonella selective medium like Tetrathionate Broth and Selenite enrichment broth and to Tetrathionate-Brilliant Green Broth for the detection of Salmonellae in milk samples. The enriched culture of Rappaport Vasiliadis Salmonella Enrichment Broth (MH1491) can be further subcultured and isolated on Xylose Lysine Deoxycholate Agar (MH031).
Type of specimen
Pharmaceutical samples.
Specimen Collection and Handling:
For pharmaceutical samples follow appropriate techniques for handling specimens as per established guidelines (3-7). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions :
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets
Limitations :
Overheating may destroy the selectivity of medium.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Light yellow to light blue coloured granular medium
Colour and Clarity of prepared medium: Greenish blue coloured clear to slightly opalescent solution with a slight precipitate in tubes.
pH: 5.00-5.40
Cultural Response: Growth Promotion is carried out in accordance with harmonized method of USP/BP/EP/JP/IP. Cultural response was observed after an incubation at 30-35°C for specified time. Recovery is carried out using Xylose Lysine Deoxycholate Agar (MH031), after enrichment in Rappaport Vassiliadis Salmonella Enrichment Broth.
Growth promoting properties: Clearly visible growth of microorganism comparable to that previously obtained with previously tested and approved lot of medium occurs at the specified temperature for not more than the shortest period of time specified inoculating <=100 cfu (at 30-35°C for <=18 hours).
Inhibitory properties: No growth of the test microorganism occurs for the specified temperature for not less than longest period of time specified inoculating >=100 cfu (at least 100 cfu) (at 30-35°C for >= 24 hours).
Cultural Response
| Organism | Inoculum (CFU) | Growth | Observed Lot Recovery value (CFU) | Colour of colony | Incubation temperature | |
|---|---|---|---|---|---|---|
| Growth promoting | ||||||
| Salmonella Typhimurium ATCC 14028 (00031*) | 50-100 | luxuriant | >=35 | >=70% | red with black centers | <=18 hrs |
| Salmonella Abony NCTC 6017 (00029*) | 50-100 | luxuriant | >=35 | >=70% | red with black centers | <=18 hrs |
| Inhibitory | ||||||
| Staphylococcus aureus subsp. aureus ATCC 6538(00032*) | >=103 | inhibited | 0 | 0% | >=24 hrs | |
| Additional Microbiological testing | ||||||
| Escherichia coli ATCC 25922 (00013*) | 50-100 | none-poor | 0-10 | 0-10% | yellow | 18-24 hrs |
| Escherichia coli ATCC 8739 (00012*) | 50-100 | none-poor | 0-10 | 0-10% | yellow | 18-24 hrs |
| Salmonella Enteritidis ATCC 13076 (00030*) | 50-100 | luxuriant | >=35 | >=70% | red with black centre | 18-24 hrs |
| Salmonella Paratyphi B ATCC 8759 | 50-100 | luxuriant | >=35 | >=70% | red with black centre | 18-24 hrs |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | >=103 | inhibited | 0 | 0% | >=24 hrs | |
| ^Pseudomonas paraeruginosa ATCC 9027 (00026*) | >=103 | inhibited | 0 | 0% | >=24 hrs | |
| Pseudomonas aeruginosa ATCC 27853(00025*) | >=103 | inhibited | 0 | 0% | >=24 hrs | |
| Enterococcus feacalis ATCC 29212 (00087*) | >=103 | inhibited | 0 | 0% | >=24 hrs | |
| E.coli +S.Typhimurium (mixed culture) | ||||||
| Salmonella Typhimurium ATCC 14028 (00031*) | 50-100 | luxuriant | >=35 | >=70% | red with black centre | 18-72 hrs |
Key: (*) Corresponding WDCM numbers. ^ Formerly known as Pseudomonas aeruginosa
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (9,10).
| Product Name | Rappaport Vassiliadis Salmonella Enrichment Broth, Granulated |
|---|---|
| SKU | GMH1491 |
| Product Type | Granulated |
| Physical Form | Granular |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Van Schothorst M., Renauld A. and VanBeek C., 1987, Food Microbiol., 4: |
| Customized Product Available | No |
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