Malt Extract Medium (without Membrane Filter)

Principle And Interpretation

Fields of Application: Water, Food, Milk and other samples. DriFilter Membrane Nutrient Pad Medium is ready to use sterile culture media in the form of a 50 mm biological inert absorbent pads impregnated with Malt Extract medium, then dried and sterilized in 55 mm petri plate. They eliminate the need of laborious media preparation and autoclaving procedures. The nutrient pads are to be just rewetted with sterile distilled water and are ready to use. Use of nutrient pads allows larger sample volumes to be tested at a time. Interpretation of results is directly by counting the CFUs and also quantifies the microbial load present in the sample. Media used for the isolation of fungi are acidic and are designed to be inhibitory to bacteria. The use of malt and malt extracts for the propagation of yeasts and moulds is quite common. Reddish (1) described a culture medium prepared from malt extract that was a satisfactory substitute for wort. Malt Extract Medium is similar to the formula of Galloway and Burgess (2) used for the detection, isolation and enumeration of yeasts and moulds. Malt Extract medium is recommended for the examination of yeasts and moulds in the U.S. Food and Drug Administrations Bacteriological Analytical Manual (2). For mycological counts it may be desirable to prepare more acidic medium in order to suppress bacterial growth. Malt extract provides an acidic environment and nutrients favorable for growth and metabolism of yeasts and moulds. Mycological peptone rapidly gives a luxuriant growth with typical morphology and pigmentation.