Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Antibiotic Assay Medium D
Availability: Out of stock
Availability: Out of stock
Availability: Out of stock
Only %1 left
SKU:
ME556
For the microbiological assay of Erythromycin and Neomycin using Klebsiella pneumoniae as a test organism in accordance with European Pharmacopoeia.
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Composition**
| Ingredients | Gms / Litre |
|---|---|
| Heart extract | 1.500 |
| Yeast extract | 1.500 |
| Peptone casein | 5.000 |
| Glucose monohydrate | 1.000 |
| Sodium chloride | 3.500 |
| Dipotassium hydrogen phosphate | 3.680 |
| Potassium dihydrogen phosphate | 1.320 |
| Potassium nitrate | 2.000 |
| pH after sterilization | 7.0±0.1 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 19.4 grams of dehydrated medium powder in 1000 ml purified/R-water/distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Adjust the pH of the medium using freshly prepared buffer solution as recommended by the European pharmacopoeia for the antibiotic assayed.
Principle And Interpretation
This medium is formulated in accordance to European Pharmacopoeia (2).This medium is widely used for turbidimetric assay of erthromycin estolate using Klebsiella pneumoniae as test organism (1). Turbidimetric methods for determining the potency of antibiotics are inherently more accurate and more precise than comparable agar diffusion procedures.
Combination of peptone-casein, heart extract and yeast extract supply nutrients and essential mineral and growth factors for enhanced microbial growth. Potassium nitrate serves as inorganic source of nitrogen for the growth of test organism. Sodium chloride maintains the osmotic equilibrium while phosphates are incorporated in the medium to provide good buffering action. Glucose monohydrate serves as the carbon and energy source for faster growth.
Turbidimetric antibiotic assay is based on the change or inhibition of growth of a test microorganism in a liquid medium containing a uniform concentration of an antibiotic. Use of this method is appropriate only when test samples are clear.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium
Light yellow coloured clear to slightly opalescent solution
Reaction
After sterilization, reaction of 1.95% w/v aqueous solution. pH: 7.0±0.1
pH
6.90-7.10
Cultural Response
ME556: Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth | Serial dilution with |
|---|---|---|---|
| Klebsiella pneumoniae ATCC 10031 | 50-100 | luxuriant | Erythromycin estolate |
Storage and Shelf Life
Store below 30°C in tightly closed container and use freshly prepared medium. Use before expiry date on the label.
| Product Name | Antibiotic Assay Medium D |
|---|---|
| SKU | ME556 |
| Customized Product Available | No |
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