Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
Your enquiry has been submitted
Something problem with the server. Try again.
Broth Medium D (Lactose Monohydrate Broth)
Availability: Out of stock
Availability: Out of stock
Availability: Out of stock
Only %1 left
SKU:
ME1003
Used for the detection of coliform bacteria in water, food, dairy products in accordance with per European Pharmacopoeia 2008.
Your enquiry has been submitted
Please login to Post an Enquiry.
Principle and Interpretation
Lactose Monohydrate Broth is used for the detection of coliform bacteria in water, foods, dairy products in accordance with per European Pharmacopoeia 2008.
Lactose Broth is is recommended by European pharmacopoeia(4) for selective pre-enrichment of Enterobacteriaceae as well as for E.coli and Salmonella in water, food and pharmaceutical products. The medium is used for detection of specified microorganisms of non-sterile products according to European Pharmacopoeia (EP). This medium is also recommended by various other pharmacopoeia (5,6,7)
Lactose Broth is also recommended by APHA in the performance and confirmation of the presumptive test for coliform bacteria in water (1), food (2), milk (3).This medium can be used as an alternate to Lauryl Sulphate Broth in the presumptive test of the MPN of standard coliforms.
Pancreatic digest of gelatin and beef extract supply essential nutrients to the organisms. Lactose monohydrate is a fermentable carbohydrate for the coliforms. Tubes of Lactose Broth are inoculated with dilutions of water or milk, etc. under test, and incubated at 35°C and examined for gas formation after 24 and 48 hours.
Members of the coliform group are defined as aerobic and facultative anaerobic gram-negative and non-sporing bacilli which ferment lactose with gas formation within 48 hours at 35°C. In testing dairy products, Lactose Broth is used only in the completed test (3). Large water samples may require double strength Lactose Broth to minimize the final volume.
Composition
| Ingredients | Gms / Litre |
|---|---|
| Pancreatic digest of gelatin | 5.000 |
| Beef extract | 3.000 |
| Lactose monohydrate | 5.000 |
| pH after sterilization (at 25°C) | 6.9±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 12.75 grams (the equivalent weight of dehydrated powder per litre) in 1000 ml purified/distilled water. Heat if necessary to dissolve the medium completely. For larger inocula (10 ml or more), concentrated medium may be prepared to account for medium dilution by the inoculum. Dispense in tubes containing inverted fermentation vial (Durhams tube) as desired. Sterilize by autoclaving at 15lbs pressure (121°C) for 15 minutes and cool immediately.
Quality Control
Appearance: Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium: Light amber coloured clear solution
Reaction: Reaction of 1.27% w/v aqueous solution after sterilization. pH : 6.9±0.2
pH: 6.70-7.10
Growth Promotion Test: As per European Pharmacopoeia.
Cultural Response
ME1003: Cultural characteristics observed after an incubation at 35-37°C for 18-48 hours.
| Organism | Inoculum (CFU) | Growth | Gas |
|---|---|---|---|
| Enterobacter aerogenes ATCC 13048 | 50-100 | luxuriant | Positive reaction |
| Enterococcus faecalis ATCC 29212 | 50-100 | luxuriant | Negative reaction |
| Escherichia coli ATCC 8739 | 50-100 | luxuriant | Positive reaction |
| Pseudomonas aeruginosa ATCC 9027 | 50-100 | luxuriant | Negative reaction |
| Salmonella Typhimurium ATCC 14028 | 50-100 | luxuriant | Negative reaction |
| Salmonella Abony NCTC 6017 | 50-100 | luxuriant | Negative reaction |
Storage and Shelf Life
Store below 30°C in tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label.
| Product Name | Broth Medium D (Lactose Monohydrate Broth) |
|---|---|
| SKU | ME1003 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. Corry J. E. L., Curtis G. D. W., and Baird R. M., Culture Media for Food Microbiology, Vol. 34, Progress in IndustrialMicrobiology, 1995, Elsevier, Amsterdam |
| Customized Product Available | No |
Write Your Own Review
Lactose HiVeg™ Broth
As low as
$36.30
Lactose Broth, Granulated
As low as
$116.60
Fluid Lactose Medium
As low as
$36.30
Lauryl Sulphate Broth (Lauryl Tryptose Broth)
As low as
$31.90
Lactose Broth
As low as
$36.30
Broth Medium D (Lactose Monohydrate Broth)
As low as
$0.00
Lactose Broth
As low as
$39.60
Brilliant Green Bile Broth, 2%
As low as
$40.70
Fluid Lactose Medium (Lactose Broth) Medium 4
As low as
$0.00
Fluid Lactose Medium
As low as
$39.60