Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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BHI HiCynth™ Agar (Brain Heart Infusion HiCynth™ Agar) (Special Infusion HiCynth™ Agar)
As low as
$48.40
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Availability: In stock
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SKU:
MCD211
A solid medium recommended for the cultivation of fastidious pathogenic bacteria, yeasts and moulds.
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Intended Use
Recommended for the cultivation of fastidious pathogenic bacteria, yeasts and moulds from samples.
Composition**
| Ingredients | g/L |
|---|---|
| HiCynth™ Peptone No.2* | 8.000 |
| HiCynth™ Peptone No.3* | 9.500 |
| HiCynth™ Peptone No.6* | 10.000 |
| Dextrose (Glucose) | 2.000 |
| Sodium chloride | 5.000 |
| Disodium hydrogen phosphate | 2.500 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.4±0.2 |
**Formula adjusted, standardized to suit performance parameters
* Chemically defined peptones
Directions
Suspend 52.0 gram in 1000 ml purified / distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix well and pour into sterile Petri plates. If desired, 20 units Penicillin and 40 µg Streptomycin per ml of medium may be added to make the medium selective for fungi.
Principle And Interpretation
BHI Agar is highly nutritious and can support luxuriant growth of wide variety of microorganisms. It can be further enriched by the addition of blood or rendered selective by adding different antibiotics (1, 2). It is a general purpose medium used for primary isolation of aerobic bacteria from specimens. Addition of 50 mg/l chloramphenicol or 40mg/1 streptomycin or a mixture of 50mg/l gentamicin and 50mg/l chloramphenicol along with 5-10% sterile defibrinated blood is often recommended for inhibition of bacteria and isolation of pathogenic systemic fungi. A mixture of cycloheximide (0.5 g/l) and chloramphenicol (0.05 g/l) is also used for selective isolation of pathogenic fungi (incubation at 25-30°C for 1-2 weeks) (3). Some fungi may be inhibited on this medium with 10% sheep blood, gentamicin and chloramphenicol (4,5,6). BHI HiCynth™ Agar is prepared by replacing animal and vegetable peptones with chemically defined peptones to avoid BSE/TSE risks associated with animal peptones.
HiCynth™ Peptone No.2, HiCynth™ Peptone No.3 and HiCynth™ Peptone No.6 used in the media, serves as sources of carbon, nitrogen, vitamins, amino acids, along with essential growth factors. Dextrose is the energy source. Sodium chloride maintains the osmotic equilibrium of the medium while disodium hydrogen phosphate buffers the medium. Defibrinated sheep blood added to the basal medium provides essential growth factors for the more fastidious fungal organisms.
Type of specimen
Food and dairy samples
Specimen Collection and Handling
For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (5,7, 8).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- As organisms differ in their nutritional requirements, some fastidious organisms may be inhibited or may show poor growth.
- Further biochemical tests must be carried out for complete identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Gelling
Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium
Basal medium: Light amber coloured clear to slightly opalescent gel. After addition of 5% v/v sterile defibrinated blood : Cherry red coloured, opaque gel forms in Petri plates.
Reaction
Reaction of 5.2% w/v aqueous solution at 25°C. pH: 7.4±0.2
pH
7.20-7.60
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours (If desired add 5% v/v sterile defibrinated blood).
| Organism | Inoculum (CFU) | Growth | Recovery | Growth w/ blood | Recovery w/ blood |
|---|---|---|---|---|---|
| Candida albicans ATCC 26790 | 50-100 | luxuriant | >=70% | luxuriant | >=70% |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | 50-100 | luxuriant | >=70% | luxuriant | >=70% |
| Streptococcus pneumoniae ATCC 6303 | 50-100 | luxuriant | >=70% | luxuriant | >=70% |
| Shigella flexneri ATCC 12022 (00126*) | 50-100 | luxuriant | >=70% | luxuriant | >=70% |
| Escherichia coli ATCC 25922 (00013*) | 50-100 | luxuriant | >=70% | luxuriant | >=70% |
Key: (*) Corresponding WDCM numbers.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (9,10).
Reference
- Roseburg T. et al, 1944, J. Infect. Dis., 74:131.
- Conant N. F., 1950, Diagnostic Procedures and Reagents, 3rd Ed., APHA Inc.
- MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. I, Williams and Wilkins, Baltimore.
- Creitz and Puckett, 1954, Am. J. Clin. Pathol., 24:1318.
- Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Eds.), 2003, Manual of Clinical Microbiology, 8th Ed., American Society for Microbiology, Washington, D.C.
- American Public Health Association, Standard Methods for the Examination of Dairy Products, 1978, 14th Ed., Washington D.C.
- Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed., APHA Inc., Washington, D.C.
- Isenberg, H.D. Clinical Microbiology Procedures Handbook 2nd Edition.
- Jorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015) Manual of Clinical Microbiology, 11th Edition. Vol. 1.
| Product Name | BHI HiCynth™ Agar (Brain Heart Infusion HiCynth™ Agar) (Special Infusion HiCynth™ Agar) |
|---|---|
| SKU | MCD211 |
| Product Type | HiCynth™ |
| Physical Form | Powder |
| Origin | Chemically defined (HiCynth™) |
| Packaging type | HDPE |
| References | 1. Roseburg T. et al, 1944, J. Infect. Dis., 74:131. 2.Conant N. F., 1950, Diagnostic Procedures and Reagents, 3rd Ed., APHA Inc. 3.MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. I, Williamsand Wilkins, Baltimore. 4.Creitz and Puckett, 1954, Am. J. Clin. Pathol., 24:1318. 5.Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Eds.), 2003, Manual of Clinical Microbiology,8th Ed., American Society for Microbiology, Washington, D.C. 6.Ajello L., Georg L., Kaplan W. and Kaufman L., 1963, CDC Laboratory Manual for Medical Mycology, PHS PublicationNo. 994, U.S. Govt. Office, Washington, D.C. 7.Isenberg, H.D. Clinical Microbiology Procedures Handb0ook. 2nd Edition. 8.Jorgensen,J.H., Pfaller , M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015) Manual ofClinical Microbiology, 11th Edition. Vol. 1. |
| Customized Product Available | No |
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