Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Bismuth Sulphite HiCynth™ Agar
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$45.10
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Availability: In stock
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SKU:
MCD027
Recommended for the selective isolation and preliminary identification of Salmonella Typhi and other Salmonellae from pathological materials such as urine, sewage, water supplies, food etc.
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Intended Use
Recommended for the selective isolation of Salmonella from sewage, water supplies, food and other materials.
Composition
| Ingredients | g/L |
|---|---|
| HiCynth™ Peptone No.2* | 10.000 |
| HiCynth™ Peptone No.6* | 5.000 |
| Dextrose (Glucose) | 5.000 |
| Disodium hydrogen phosphate | 4.000 |
| Ferrous sulphate | 0.300 |
| Bismuth sulphite indicator | 8.000 |
| Brilliant green | 0.025 |
| Agar | 20.000 |
| Final pH (at 25°C) | 7.7±0.2 |
**Formula adjusted, standardized to suit performance parameters
*Chemically defined peptones
Directions
Suspend 52.33 gram in 1000 ml purified / distilled water. Heat to boiling to dissolve the medium completely. DO NOT STERILIZE IN AUTOCLAVE or by fractional sterilization since overheating may destroy the selectivity of the medium. The sensitivity of the medium depends largely upon uniform dispersion of precipitated bismuth sulphite in the final gel, which should be dispersed before pouring into sterile Petri plates.
Principle And Interpretation
The Salmonella constitute the most taxonomically complex group of bacteria among Enterobacteriaceae (1). Human Salmonella infections are most commonly caused by ingestion of food, water or milk contaminated by human or animal excreta. Humans are the only reservoirs of S. Typhi (2). Of the various media employed for the isolation and preliminary identification of Salmonella, particularly Salmonella Typhi; Bismuth Sulphite Agar is the most productive (3). This medium is prepared by replacing animal and vegetable peptones with chemically defined peptones to avoid BSE/TSE risks associated with animal peptones.
Bismuth Sulphite Agar is a modification of original Wilson and Blair Medium (4-6). It is also recommended by various Associations (2,7-10) for the isolation and preliminary identification of Salmonella Typhi and other Salmonella from pathological materials, sewage, water, food and other products.
S.Typhi, S. Enteritidis and S. Typhimurium typically grow as black colonies with a surrounding metallic sheen resulting from hydrogen sulphide production and reduction of sulphite to black ferric sulphide. Salmonella Paratyphi A grows as light green colonies. Bismuth Sulphite Agar may be inhibitory to some strains of Salmonella species and therefore should not be used as the sole selective medium for these organisms. Also this medium favors use of larger inoculum as compared to other selective media, as it has unique inhibitory action towards gram-positive organisms and coliforms.
HiCynth™ Peptone No.2 and HiCynth™ Peptone No.6 serve as sources as carbon, nitrogen, long chain amino acids, vitamins and essential growth factors. Dextrose is the carbon source. Disodium phosphate maintains the osmotic equilibrium. Bismuth sulphite indicator along with brilliant green inhibits the intestinal gram-positive and gram-negative bacteria. Ferrous sulphate aids in detection of hydrogen sulphide production. In case of food samples, pre enrichment of the sample is done prior to inoculation.
Type of specimen
Food and water samples.
Specimen Collection and Handling
For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (10,11,12). For water samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards (2). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- DO NOT AUTOCLAVE OR OVERHEAT THE MEDIUM, as it destroys the selectivity of the medium.
- S.Typhi and S. Arizonae exhibit typical brown colonies, with or without metallic sheen.
- This medium is highly selective and must be used in parallel with less selective media for isolation.
- With certain Salmonella species, typical black colonies with metallic sheen is observed near heavy inoculation and isolated colonies may show green colonies.
- Shigella species are mostly inhibited on this medium; exceptions being S. flexneri and S. sonnei (8).
- Some Salmonella like S. Sendai, S. Berta, S. Gallinarum, S. Abortus-equi are also inhibited (8).
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Light yellow to greenish yellow homogeneous free flowing powder
Gelling
Firm, comparable with 2.0% agar gel.
Colour and Clarity of prepared medium
Greenish yellow coloured, opalescent with flocculent precipitate forms in Petri plates.
Reaction
Reaction of 5.23% w/v aqueous solution at 25°C. pH : 7.7±0.2
pH
7.50-7.90
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 40-48 hours.
| Organism | Inoculum (CFU) | Growth | Recovery | Colour of Colony |
|---|---|---|---|---|
| # Klesiella aerogenes ATCC 13048 (00175*) | 50-100 | none-poor | <=10% | brown-green (depends on the inoculum density) |
| Enterococcus faecalis ATCC 29212 (00087*) | >=104 | inhibited | 0% | |
| Escherichia coli ATCC 25922 (00013*) | 50-100 | none-poor | <=10% | brown-green (depends on the inoculum density) |
| Salmonella Enteritidis ATCC 13076 (00030*) | 50-100 | good-luxuriant | >=50% | black with metallic sheen |
| Salmonella Typhi ATCC 6539 | 50-100 | good-luxuriant | >=50% | black with metallic sheen |
| Salmonella Typhimurium ATCC 14028 (00031*) | 50-100 | good-luxuriant | >=50% | black with metallic sheen |
| Shigella flexneri ATCC 12022 (00126*) | 50-100 | none-poor | <=10% | brown |
| Escherichia coli ATCC 8739 (00012*) | 50-100 | none-poor | <=10% | brown to green, depends on inoculum density |
| Salmonella Abony NCTC 6017 (00029*) | 50-100 | good-luxuriant | >=50% | black with metallic sheen |
Key: (*) Corresponding WDCM numbers. (#) Formerly known as Enterobacter aerogenes
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (13,14).
| Product Name | Bismuth Sulphite HiCynth™ Agar |
|---|---|
| SKU | MCD027 |
| Product Type | HiCynth™ |
| Physical Form | Powder |
| Origin | Chemically defined (HiCynth™) |
| Packaging type | HDPE |
| References | 1. Tindall B. J., Crimont P. A. D., Gorrity G. M., EUZESY B. P., 2005, Int. J. Sys. Evol. Microbiol., 55:521 2.Koneman E. W., Allen S. D., Janda W. M., Schreckenberger P. C., Winn W. C. Jr., 1992, Colour Atlas and Textbook ofDiagnostic Microbiology, 4 th Ed., J. B. Lippinccott Company 3.Mandell G. L., Douglas R. G. Jr., Bennet J. E., (Eds.) , 1985, Principles and Practice of Infectious Diseases, 2nd Ed., 660-669,John Wiley & Sons New York. 4.Gunter and Tuft, 1939, J. Lab. Clin. Med., 24:461. 5.Wilson and Blair, 1926, J. Pathol. Bateriol., 29:310. 6.Wilson and Blair, 1927, J. Hyg., 26:374 9.Baird R.B., Eaton A.D., and Rice E.W., (Eds.), 2015, Standard Methods for the Examination of Water andWastewater, 23rd ed., APHA, Washington, D.C. 10.FDA Bacteriological Analytical Manual, 2005, 18th Ed., AOAC, Washington, D.C. 11. Murray P. R., Baron J. H., Pfaller M. A., Tenover F. C. and Yolken R. H., (Eds.). 1999, Manual of Clinical Microbiology,7th Ed., American Society for Microbiology, Washington, D.C. 12.Salfinger Y., and Tortorello M.L. Fifth (Ed.), 2015, Compendium of Methods for the Microbiological Examination ofFoods, 5th Ed., American Public Health Association, Washington, D.C.1 3.Indian Pharmacopoeia, 1996, Ministry of Health and Family Welfare, Govt. of India, Volume 2.1 4.MacFaddin J. F., 2000, (Ed.), Biochemical Tests for Identification of Medical Bacteria, 3rd Edition, Lippincott, Williamss& Wilkins, New York.1 5.American Public Health Association, Standard Methods for the Examination of Dairy Products, 1978, 14th Ed.,Washington D.C.1 6.Isenberg, H.D. Clinical Microbiology Procedures Handb0ook. 2nd Edition.1 |
| Customized Product Available | No |
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