Hi-SYBr Master Mix (with Taq Polymerase and Low-ROX), supplied in 2X concentration, is a ready-to-use mix convenient for Real-Time PCR. The master mix contains: 1.SYBr Green Dye 2.Low-ROX as Internal Reference Dye 3. Taq polymerase ? dNTPs ? Assay buffer 4. MgCl2 Template, primers and nuclease?free watershould be added before setting up the PCR reaction. Asthe mixture is ready-to-use, the reaction set time is reduced to half.