Hi-SYBr Master Mix (with Taq Polymerase and High ROX), supplied in 2X concentration, is a ready-to-use mix convenient for Real-Time PCR.
The master mix contains:
1.SYBr Green Dye
2.ROX as Internal Reference Dye
3.Taq polymerase
4.dNTPs
5.Assay buffer
6.MgCl2
Template, primers and nuclease-free water should be added before setting up the PCR reaction. As the mixture is ready-to-use, the reaction set time is reduced to half