Hi-Temp PCR Master Mix

Hi-Temp PCR Master Mix is a premixed, ready-to-use solution containing Hi-Temp DNA polymerase, dNTPs, MgCl2 and reaction buffers at optimal concentrations for efficient amplification of DNA templates by PCR. This pre-mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up. The mix is optimized for efficient and reproducible PCR. Hi-Temp DNA Polymerase is a complex of specific anti-Taq monoclonal antibody with best quality thermostable Taq DNA Polymerase for automatic “hot start” amplification, resulting in greatly enhanced amplification specificity, sensitivity and yield. Hi-Temp DNA Polymerase catalyzes the polymerization of nucleotides into duplex DNA in the 5’-3’direction in the presence of Mg2+ and has the 5’-3’ exonuclease activity. Hi-Temp DNA polymerase in the master mix is inactivated with monoclonal antibodies that bind the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94°C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.