HiPurA® DH5α Competent Cells

HiPurA® Competent Cells are specially prepared chemically competent DH5α cells which makes cloning experiments easier. Competent cells are ready to use bacterial cells that contain an altered cell membrane through which a foreign DNA can pass through very easily. HiPurA® Competent Cells are prepared by following a modified protocol of Hanahan (1983, J.Mol.Biol 166:557) and are recommended for regular subcloning of recombinant plasmids. DH5α is the most frequently used E. coli strain for routine cloning applications through blue/white screening of colonies on plates containing X-Gal and IPTG as the lacZ∆M15 marker provides α-complementation of the β-galactosidase gene. A stock pUC19 solution is provided as a control to determine the transformation efficiency. To obtain maximum transformation efficiency, the experimental DNA must be free of phenol, ethanol, protein and detergents. HiPurA® DH5A Competent Cells are chemically treated Escherichia coli strains prepared for high frequency Bacterial Transformation. These Cloning Cells undergo Chemical Competence Induction to facilitate Recombinant DNA Vector Propagation in routine molecular biology.