Restriction enzymes are essential tools in molecular biology because each recognizes a unique DNA sequence and precisely cuts the double-stranded DNA molecule into smaller, distinct fragments.
Sma I is a Restriction Endonuclease purified from an Serratia marcescens strain that carries the Sma I gene.
The restriction enzyme Sma I recognizes the DNA sequence C C C^G G G GAATTC and optimally cleaves the DNA at 37°C when used with 10X Assay Buffer