Restriction enzymes are essential tools in molecular biology because each recognizes a unique DNA sequence and precisely cuts the double-stranded DNA molecule into smaller, distinct fragments. Nco I is a Restriction Endonuclease purified from an Nocardia corallina strain that carries the Nco I gene. The restriction enzyme Nco I recognizes the DNA sequence C^C A T G G and optimally cleaves the DNA at 37°C when used with 10X Assay Buffer. Nco I is a restriction enzyme that recognizes a sequence often containing an ATG start codon. The Endonuclease Activity targets the CCATGG Recognition Sequence during a Restriction Digest for Protein Expression Vector Cloning and translational fusion research.