Stu I

Restriction enzymes are essential tools in molecular biology because each recognizes a unique DNA sequence and precisely cuts the double-stranded DNA molecule into smaller, distinct fragments. Stu I is a Restriction Endonuclease purified from an Streptomyces tubercidicus strain that carries the Stu I gene. The restriction enzyme Stu I recognizes the DNA sequence A G G^C C T and optimally cleaves the DNA at 37°C when used with 10X Assay Buffer. Stu I is an endonuclease that cleaves DNA to produce fragments with non cohesive termini. This Restriction Enzyme targets the AGGCTT Recognition Sequence for DNA Digestion and facilitates Blunt End DNA Ligation for genomic library construction and cloning.