Sac I

Restriction enzymes are essential tools in molecular biology because each recognizes a unique DNA sequence and precisely cuts the double-stranded DNA molecule into smaller, distinct fragments. Sac I is a Restriction Endonuclease purified from an Streptomyces achromogenes strain that carries the Sac I gene. The restriction enzyme Sac I recognizes the DNA sequence G A G C T^C and optimally cleaves the DNA at 37°C when used with 10X Assay Buffer. Sac I is a high purity endonuclease designed for site specific cleavage of double stranded DNA. This Restriction Enzyme ensures GAGCTC Recognition Sequence targeting to facilitate a Molecular Cloning Procedure and the assembly of recombinant genetic constructs.