Restriction enzymes are essential tools in molecular biology because each recognizes a unique DNA sequence and precisely cuts the double-stranded DNA molecule into smaller, distinct fragments.
Sac I is a Restriction Endonuclease purified from an Streptomyces achromogenes strain that carries the Sac I gene.
The restriction enzyme Sac I recognizes the DNA sequence G A G C T^C and optimally cleaves the DNA at 37°C when used with 10X Assay Buffer