Restriction enzymes are essential tools in molecular biology because each recognizes a unique DNA sequence and precisely cuts the double-stranded DNA molecule into smaller, distinct fragments.
Pst I is a Restriction Endonuclease purified from an Providencia stuartii strain that carries the Pst I gene.
The restriction enzyme Pst I recognizes the DNA sequence C T G C A^G and optimally cleaves the DNA at 37°C when used with 10X Assay Buffer