Restriction enzymes are essential tools in molecular biology because each recognizes a unique DNA sequence and precisely cuts the double-stranded DNA molecule into smaller, distinct fragments.
Not I is a Restriction Endonuclease purified from an Nocardia otitidis strain that carries the Not I gene.
The restriction enzyme Not I recognizes the DNA sequence G C^G G C C G C and optimally cleaves the DNA at 37°C when used with 10X Assay Buffer