Restriction enzymes are essential tools in molecular biology because each recognizes a unique DNA sequence and precisely cuts the double-stranded DNA molecule into smaller, distinct fragments.
EcoRI is a Restriction Endonuclease purified from an Escherichia coli strain that carries the EcoRI gene.
The restriction enzyme EcoRI recognizes the DNA sequence G^AATTC and optimally cleaves the DNA at 37°C when used with 10X Assay Buffer