HiMedia's HIS-Tagged Bacterial Protein Purification Kit (Spin Column) is used for rapid purification of recombinant proteins fused to 6X HIS and expressed in bacteria. The kit contains pre-packed ready-to-use columns of 0.2 ml Ni-NTA Agarose resin in 30% ethanol. HIS-Tagged Bacterial Protein Purification Kit (Spin Column) employs immobilized metal affinity chromatography for the rapid isolation of polyhistidine fused proteins. This kit facilitates HIS tagged protein purification for molecular recombinant protein research using a convenient centrifugal format for small scale processing.
Technical Data Sheet (TD)
HIS-Tagged Bacterial Protein Purification Kit (Spin Column)
Safety Data Sheet (SDS)
HIS-Tagged Bacterial Protein Purification Kit (Spin Column)
Electronic Instructions for Use (eIFU)
HIS-Tagged Bacterial Protein Purification Kit (Spin Column)
HIS-Tagged Bacterial Protein Purification Kit (Spin Column)
HIS-Tagged Bacterial Protein Purification Kit (Spin Column) is used for purification of histidine-tagged proteins using spin column. This kit conatins Ni-NTA Agarose; nitrilotriacetic acid (NTA), a tetradentate chelating ligand, in a highly
cross-linked 6% agarose matrix. It is used for purification of polyhistidine-tagged recombinant
proteins expressed in Escherichia coli having an affinity for nickel metal ion. The kit contains pre-packed ready-to-use columns of 2 ml Ni-NTA Agarose resin in 30% ethanol having binding capacity of 50 mg of HIS-tagged protein/ml resin.