HiMedia's HIS-Tagged Bacterial Protein Purification Kit (Gravity Flow) is used for rapid purification of recombinant proteins fused to 6X HIS and expressed in bacteria. The kit contains pre-packed ready-to-use columns of 2 ml Ni-NTA Agarose resin in 30% ethanol. HIS-Tagged Bacterial Protein Purification Kit (Gravity Flow) utilizes immobilized metal affinity chromatography to capture polyhistidine fused proteins. This kit enables HIS tagged protein purification for molecular recombinant protein research and structural biology studies.
Technical Data Sheet (TD)
HIS-Tagged Bacterial Protein Purification Kit (Gravity Flow)
Safety Data Sheet (SDS)
HIS-Tagged Bacterial Protein Purification Kit (Gravity Flow)
Electronic Instructions for Use (eIFU)
HIS-Tagged Bacterial Protein Purification Kit (Gravity Flow)
HIS-Tagged Bacterial Protein Purification Kit (Gravity Flow)
HIS-Tagged Bacterial Protein Purification Kit (Gravity Flow) is used for purification of histidine-tagged proteins using gravity flow. This kit conatins Ni-NTA Agarose; nitrilotriacetic acid (NTA), a tetradentate chelating ligand, in a highly
cross-linked 6% agarose matrix. It is used for purification of polyhistidine-tagged recombinant
proteins expressed in Escherichia coli having an affinity for nickel metal ion. The kit contains pre-packed ready-to-use columns of 2 ml Ni-NTA Agarose resin in 30% ethanol having binding capacity of 50 mg of HIS-tagged protein/ml resin.