HIS-Tagged Bacterial Protein Purification Kit (Gravity Flow) is used for purification of histidine-tagged proteins having an affinity for nickel using gravity flow having binding capacity of 50 mg HIS-tagged protein/ml resin.
Technical Data Sheet (TD)
HIS-Tagged Bacterial Protein Purification Kit (Gravity Flow)
Safety Data Sheet (SDS)
HIS-Tagged Bacterial Protein Purification Kit (Gravity Flow)
Electronic Instructions for Use (eIFU)
HIS-Tagged Bacterial Protein Purification Kit (Gravity Flow)
HIS-Tagged Bacterial Protein Purification Kit (Gravity Flow)
HIS-Tagged Bacterial Protein Purification Kit (Gravity Flow) is used for purification of histidine-tagged proteins using gravity flow. This kit conatins Ni-NTA Agarose; nitrilotriacetic acid (NTA), a tetradentate chelating ligand, in a highly
cross-linked 6% agarose matrix. It is used for purification of polyhistidine-tagged recombinant
proteins expressed in Escherichia coli having an affinity for nickel metal ion. The kit contains pre-packed ready-to-use columns of 2 ml Ni-NTA Agarose resin in 30% ethanol having binding capacity of 50 mg of HIS-tagged protein/ml resin.