The IgG Purification Kit (Protein G Based, Spin Column) is based on the affinity of IgG to immobilized Protein G agarose and is used for purification of polyclonal antibodies. When a suspension (e.g. serum, ascites fluid, tissue culture supernatant) containing mixture of substances along with IgG are loaded on the column, the IgG binds to Protein G and is recovered by elution. IgG Purification Kit (Protein G Based, Spin Column) provides a high speed format for the recovery of specific immunoglobulin fractions. It facilitates IgG purification and centrifugal antibody recovery using Protein G media for molecular diagnostic assay development and protein characterization.
Technical Data Sheet (TD)
IgG Purification Kit (Protein G Based,
Spin Column)
Safety Data Sheet (SDS)
IgG Purification Kit (Protein G Based,
Spin Column)
Electronic Instructions for Use (eIFU)
IgG Purification Kit (Protein G Based,
Spin Column)
IgG Purification Kit (Protein G Based,
Spin Column)
The IgG Purification Kit (Protein G Based, Gravity Flow) is based on the affinity of IgG to
immobilized Protein G agarose and is used for purification of polyclonal antibodies. Protein G is a genetically engineered recombinant protein
with three IgG-binding regions of native Protein G and covalently immobilized onto 4% cross-linked agarose. It is ideal for polyclonal IgG purification from mouse, human, cow, goat and sheep
serum, including human IgG3 and mouse IgG1 isotypes utilizing spin column technique. It has binding capacity of Approximately 20 mg human IgG/ml resin