IgG Purification Kit (Protein A Based, Gravity Flow)

The IgG Purification Kit (Protein A Based, Gravity Flow) is based on the affinity of IgG to immobilized Protein A agarose and is used for purification of polyclonal antibodies. Protein A contains five homologous antibody–binding domains with high affinity for subclasses of IgG from many species and for some IgA and IgM. it is used for the Purification of IgG fractions (from crude serum, ascites fluid, tissue culture supernatant) and for Isolation of antigen-antibody complex (during immunoprecipitation) based on gravity flow technique. The kit contains pre-packed ready-to-use columns of 2 ml Protein A agarose resin in 20% ethanol with a binding capacity of 25mg human IgG /ml of resin.