The Hi‐PCR® 18S rRNA SYBr PCR Kit is designed for the detection of a specific sequence of the 18S rRNA gene in food, clinical samples, environmental samples etc. The 18S rRNA specific primers generate an amplicon of 182 bp. This kit also contains Positive control. Hi-PCR® 18S rRNA SYBr PCR Kit provides universal identification of eukaryotic organisms by monitoring fluorescent signal levels. This kit supports 18S rRNA recovery for eukaryotic metagenomic screening and molecular parasite research in various ecosystems.
Eukaryotic 18S ribosomal RNA (rRNA) gene primers that feature a wide coverage are critical in detecting the composition of eukaryotic microscopic organisms in ecosystems. Here, 18S rRNA primers based on consecutive conserved sites and evaluated their coverage efficiency and scope of application to different eukaryotic groups. As PCR is being used increasingly as a diagnostic tool, common conserved regions in eukaryotic 18S rRNA sequences serve as target in PCR assays for better characterization of samples. The Hi-PCR® 18S rRNA SYBr PCR Kit is designed to specifically amplify a region within the 18S rRNA.