Alkaline Hemoglobin Electrophoresis Kit is intended for discrimination of hemoglobin variants on agarose gel using alkaline buffer. Each hemoglobin molecule, normal as well as abnormal variant has a particular distinct charge on basis of which it can be separated on agarose gel. Use of Agarose, special, low EEO (DS0205) is recommended for separation of hemoglobin molecule components. Red Blood Cells are pelleted down by centrifuging blood sample and these RBC’s are further lysed to separate out hemoglobin from them. The isolated hemoglobin is then loaded into Agarose, special, low EEO (DS0205) and electrophoresed at high voltage and high current.
Technical Data Sheet (TD)
Alkaline Hemoglobin Electrophoresis Kit (No Destaining Required)
Safety Data Sheet (SDS)
Alkaline Hemoglobin Electrophoresis Kit (No Destaining Required)
Electronic Instructions for Use (eIFU)
Alkaline Hemoglobin Electrophoresis Kit (No Destaining Required)
Alkaline Hemoglobin Electrophoresis Kit (No Destaining Required)
Red blood cells (RBC’s) deliver oxygen to cells throughout the body. Hemoglobin is an oxygen?
carrying protein within the red blood cells. Sickle cell disease is a group of genetic disorders
having effect on hemoglobin. In sickle cell disease the RBC’s become crescent or sickle shaped.
These cells have a short life span as compared to normal, round red blood cells, which lead to
anemia.Anemia can cause shortness of breath, fatigue, and delayed growth and development in
children. The rapid breakdown of red blood cells may also cause yellowing of the eyes and skin,
which are signs of jaundice .Alkaline Hemoglobin Electrophoresis Kit is intended for discrimination of hemoglobin variants on agarose gel using alkaline buffer. Each hemoglobin molecule, normal as well as abnormal variant has a particular distinct charge on basis of which it can be separated on agarose gel. Use of Agarose, special, low EEO (DS0205) is recommended for separation of hemoglobin molecule components. Red Blood Cells are pelleted down by centrifuging blood sample and these RBC’s are further lysed to separate out hemoglobin from them. The isolated hemoglobin is then loaded into Agarose, special, low EEO (DS0205) and electrophoresed at high voltage and high current.