HiPurA® Insect DNA Purification Kit

HiPurA® Insect DNA Purification Kit provides a fast and easy method for purification of total DNA from insects, arthropods, roundworms, flatworms, etc using the miniprep spin column. The method is suitable for fresh as well as frozen insect samples. Insects are ground in liquid nitrogen using a mortar and pestle and subjected to lysis by Porteinase K in salt solution, followed by adsorption of DNA to the membrane, removal of residual contaminants and elution of pure genomic DNA. adsorption of DNA to the membrane, removal of residual contaminants and elution of pure genomic DNA. Concentration of recovered DNA can be estimated by agarose gel electrophoresis. Typically 50 mg of tissue yields upto 5-15 µg of DNA.