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HiPurA® Endotoxin free Plasmid DNA Midiprep Purification Kit
HiPurA® Endotoxin free Plasmid DNA Midiprep Purification Kit provides a fast and easy method for purification of total DNA for reliable applications in PCR, library screening and sequencing. The DNA purification procedure using the midiprep spin-columns comprises of
three steps viz. adsorption of DNA to the membrane, removal of residual contaminants andelution of pure plasmid DNA. HiMedia’s HiPure Midiprep spin-column format allows rapid processing of multiple samples. The columns have a high binding capacity and high-quality
DNA is obtained. The DNA obtained is compatible with downstream applications such as restriction enzyme digestion, ligation, PCR and sequencing.
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The HiPurA® Endotoxin free Plasmid DNA Midiprep Purification Kit integrates an efficient endotoxin removal step into plasmid purification procedure. Cells are lysed and endotoxins are selectively precipitated in a specialized buffer. The resultant lysate is then applied onto the silica column for binding of the DNA molecules in the presence of high salt concentration. The adsorbed DNA is washed to remove contaminants, and the pure endotoxin free plasmid DNA is eluted in Elution Buffer. The purified plasmid DNA can be used for immediate use in all molecular biology procedures such as transfection, automated sequencing and other downstream applications. The kit utilizes an advanced silica-based membrane technology in
the form of a convenient spin-column, which helps to recover upto 25-100 µg of high- or lowcopy plasmid DNA from upto 150 ml of E.coli culture per isolation procedure. The endotoxin levels are efficiently reduced to less than 0.1 EU/µg.
| Product Name | HiPurA® Endotoxin free Plasmid DNA Midiprep Purification Kit |
|---|---|
| SKU | MB514 |
| Customized Product Available | No |
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