Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Mutans-Sanguis Agar
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SKU:
M977
Recommended for differentiation of Streptococcus mutans and Streptococcus sanguis associated with oral microflora.
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Intended Use
Recommended for differentiation of Streptococcus mutans and Streptococcus sanguinis associated with oral microflora.
Composition
| Ingredients | g/L |
|---|---|
| Tryptone | 15.000 |
| Yeast extract | 5.000 |
| L-Cystine | 0.200 |
| Sodium sulphite | 0.100 |
| Sodium chloride | 1.000 |
| Disodium hydrogen phosphate | 0.800 |
| Sodium bicarbonate | 2.000 |
| Sodium acetate | 12.000 |
| Saccharose (Sucrose) | 50.000 |
| Agar | 12.000 |
Final pH (at 25°C) 7.3±0.2
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 98.1 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix well and pour into sterile Petri plates.
Principle And Interpretation
Streptococcus mutans is gram-positive, facultatively anaerobic bacteria commonly found in the human oral cavity and is a significant contributor to tooth decay. They metabolize sucrose to lactic acid (1). Sucrose is the only sugar that S. mutans can utilize. S. mutans is found in dental plaque, in blood, on heart valves in subacute endocarditis, and infrequently in saliva and throat specimens. Streptococcus sanguis is also a part of oral flora and preferentially colonize the tooth surface (2). Mutans Sanguis Agar is recommended for differentiation of S. mutans and S. sanguis.
Tryptone, yeast extract and L-cystine in the medium provide nitrogen, vitamins and minerals necessary to support bacterial growth. Sodium sulphite, sodium acetate, disodium phosphate, and sodium bicarbonate are sources of ions that simulate metabolism. Mutans Sanguis Agar contains sucrose, which allows some species of Streptococci to produce characteristic colonies as a result of extracellular polysaccharide formation from this substrate. S. mutans forms rough, heaped, irregular colonies resembling frosted glass. Mostly crumbly, although whole colonies can be picked off the agar which are white, grey or yellow in colour and 0.5 - 2 mm in diameter, may produce a drop of liquid (water-soluble glucan) on top of the colony or a puddle of polysaccharide around the colony. Streptococcus sanguis forms smooth or rough, hard and rubbery colonies, which adhere strongly to the agar making them difficult to remove with a loop. They are grey, white or colourless, 1-3 mm in diameter. Some strains do not produce extracellular polysaccharide.
Type of specimen
Clinical samples : throat swab, saliva secretions, etc.
Specimen Collection and Handling
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (3,4). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro diagnostic Use only. For professional use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
- Further biochemical and serological tests must be carried out for further identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Gelling
Firm, comparable with 1.2% Agar gel.
Colour and Clarity of prepared medium
Light yellow coloured clear to slightly opalescent gel forms in Petri plates
Reaction
Reaction of 9.81% w/v aqueous solution at 25°C. pH: 7.3±0.2
pH
7.10-7.50
Cultural Response
Cultural characteristics observed in presence of 10% CO2 + 90% H2, after an incubation at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth | Recovery | Colour of colony |
|---|---|---|---|---|
| Streptococcus mutans ATCC 25175 | 50-100 | good-luxuriant | >=50% | grayish yellow |
| Streptococcus sanguinis ATCC 10556 | 50-100 | good-luxuriant | >=50% | white, grey or colourless |
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (3,4).
Reference
- Loesche W. J., 1996, Microbiology of Dental Decay and Periodontal Disease. In: Barons Medical Microbiology (Baron S et al, eds.), 4th Ed., University of Texas Medical Branch
- Hardie J. M., Whiley R. A., 1992, The genus Streptococcus in: Balows A., Truper H. G., Dworkin M., Harder W., Schleifer K. H., (Ed.),1992, The Prokaryotes, A Handbook on the Biology of Bacteria : Ecophysiology, Isolation, Identification, Applications, 2nd Ed., Vol.II, Springer-Verlag, New York Inc.
- Isenberg, H.D. Clinical Microbiology Procedures Handbook 2nd Edition.
- Jorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015) Manual of Clinical Microbiology, 11th Edition. Vol. 1.
| Product Name | Mutans-Sanguis Agar |
|---|---|
| SKU | M977 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. Loesche W. J., 1996, Microbiology of Dental Decay and Periodontal Disease. In: Barons Medical Microbiology (Baron Set al, eds.), 4th Ed., University of Texas Medical Branch |
| Customized Product Available | No |
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