Potassium Cyanide Broth Base w/o KCN is recommended for differentiation of the members of Enterobacteriaceae on the basis of potassium cyanide tolerance. This medium is used for the differentiation of members of Enterobacteriaceae on the basis of Potassium Cyanide tolerance. Potassium Cyanide Broth Base was originally formulated by Moeller and Kauffman and Moeller. A heavy inoculum should be avoided while utilizing KCN Broth as the inoculum itself causes turbidity and / or a sediment at the bottom of the tube, which may be interpreted as false positive result . Reactions observed in KCN Broth are not sufficient to speciate organism; additional biochemical and serological tests are required.
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