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Motility Test Medium (Edwards and Ewing)

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M930S
For testing motility of enteric bacteria as recommended by BIS committee under the specifications IS:5887(Part I and V)-1976.

Motility Test Medium (Edwards and Ewing) is used for testing motility of enteric bacteria. It is recommended by BIS Committee under the specifications IS:5887(Part I and V)-1976.

Composition**

Ingredients Gms / Litre
Peptic digest of animal tissue 10.000
Meat extract 3.000
Sodium chloride 5.000
Agar 4.000
Final pH (at 25°C) 7.5±0.1

**Formula adjusted, standardized to suit performance parameters

Directions

Suspend 22 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Dispense 8 ml amounts in test tubes and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool the tubed medium in an upright position.

Principle And Interpretation

Motility Test Medium is formulated as per Edward and Ewing(1). It is recommended by BIS for testing of motility of Escherichia coli (2) and Vibrio cholerae and Vibrio parahaemolyticus (3). Motility media containing agar concentrations higher than 0.3%, produce gels through which many motile organisms cannot spread. Motile organisms spread out from the line of inoculation, while non-motile organisms grow only along the stab line.

The tubes are inoculated by stabbing with a straight wire. Motility is visualized as diffused growth away from line of inoculation. To enhance the visibility of bacterial growth 2,3,5 Triphenyl Tetrazolium Chloride (TTC) (FD057) may be added. Tetrazolium salts are colourless but are converted into insoluble formazan, a red coloured complex by the reducing properties of growing bacteria. In the Motility Test Medium containing tetrazolium, the development of this red colour helps to trace the spread of bacteria from the inoculation line. The motility of Listeria monocytogenes is frequently best observed in medium without TTC.

Quality Control

Appearance: Yellow coloured homogeneous free flowing powder

Gelling: Semisolid, comparable with 0.4% Agar gel.

Colour and Clarity of prepared medium: Yellow coloured clear gel form in tubes as butts.

Reaction: Reaction of 2.2% w/v aqueous solution at 25°C. pH : 7.5±0.1

pH: 7.40-7.60

Cultural Response: M930S: Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.

Organism Growth Motility
Enterobacter aerogenes ATCC 13048 luxuriant Positive, Growth away from stabline causing turbidity
Escherichia coli ATCC 25922 luxuriant Positive, Growth away from stabline causing turbidity
Klebsiella pneumoniae ATCC 13883 luxuriant Negative, Growth along the stabline, surrounding medium remains clear
Salmonella Enteritidis ATCC 13076 luxuriant Positive, Growth away from stabline causing turbidity
Staphylococcus aureus ATCC 25923 luxuriant Negative, Growth along the stabline, surrounding medium remains clear
Vibrio cholerae ATCC 15748 luxuriant Positive, Growth away from stabline causing turbidity
Vibrio parahaemolyticus ATCC 17802 luxuriant Positive, Growth away from stabline causing turbidity

Storage and Shelf Life

Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.

Reference

  1. Edward P.R. and Ewing W.H. 1972, Cited from, Colour Atlas and Textbook of Diagnostic Microbiology,1992, 4th ed., J.B. Lippincott Co. Philadelphia.
  2. Bureau of Indian Standards, IS : 5887 (Part - I) 1976, reaffirmed 1986.
  3. Bureau of Indian Standards, IS : 5887 (Part - V) 1976, reaffirmed 1986.
  4. Howard B. J. and Other (Eds.), 1994, Clinical and Pathogenic Microbiology, The C. V. Mosby. Year Book, Inc.
  5. Baron. E. J. and Finegold S. M. (Eds.), 1990, Bailey and Scott's 'Diagnostic Microbiology, 8th ed., The C. V. Mosby. Co, St., Louis, Missouri.
More Information
Product Name Motility Test Medium (Edwards and Ewing)
SKU M930S
Product Type Regular
Physical Form Powder
Origin Animal
Packaging type HDPE
References 1. Koneman E. W., Allen S. D., Janda W. M., Schreckenberger P. C., Winn W. C. Jr., (Eds.), 1992, Colour Atlas and Textbookof Diagnostic Microbiology, 4th Ed., J. B. Lippinccott Company.2.Tittsler R. P. and Sandholzer L. A., 1936, J. Bacteriol., 31:575.3.Edward P. R. and Ewing W. H. 1972, Identification of Enterobacteriaceae 3rd Ed., Minneapolis, Burgess.2.,,4.Howard B. J. and Other (Eds.), 1994, Clinical and Pathogenic Microbiology, The C. V. Mosby. Year Book, Inc.5.Baron. E. J. and Finegold S. M. (Eds.), 1990, Bailey and Scott's `Diagnostic Microbiology, 8th ed., The C. V. Mosby. Co,St., Louis, Missouri.6.DAmato R. F., and Tomfohrede K. M., 1981, J. Clin. Microbiol., 14 (3), 347-348.7.MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williamsand Wilkins, Baltimore.
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