Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Kanamycin Esculin Azide Broth Base
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$126.50
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SKU:
M776A
Recommended for isolation and identification of group D Streptococci in food stuffs.
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Intended Use
Recommended for selective isolation and identification of group D Streptococci in foodstuffs.
Composition**
| Ingredients | Gms/Litre |
|---|---|
| Tryptone | 20.000 |
| Yeast extract | 5.000 |
| Sodium chloride | 5.000 |
| Sodium citrate | 1.000 |
| Esculin | 1.000 |
| Ferric ammonium citrate | 0.500 |
| Sodium azide | 0.150 |
| Final pH (at 25°C) | 7.0±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 16.32 grams in 500 ml purified / distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C and aseptically add rehydrated contents of one vial of Kanamycin Sulphate Selective Supplement (FD146). Mix well and dispense into sterile tubes or flasks or as desired.
Principle And Interpretation
Kanamycin Esculin Azide Broth Base is recommended for isolation and identification of group D Streptococci in food stuffs (3). Tryptone and yeast extract provide essential growth nutrients for enterococci. Sodium azide inhibits gram negative organisms. Kanamycin has inhibitory effect on other gram-positive bacteria. Streptococci hydrolyse esculin to esculetin and dextrose. Esculetin and ferric ammonium citrate forms dark brown to black complex importing black colour to the broth.
Type of specimen
Food samples
Specimen Collection and Handling
For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (4). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred to individual safety data sheets.
Limitations :
- Further biochemical and serological tests must be carried out for complete identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Light yellow coloured homogeneous free flowing powder
Colour and Clarity of prepared medium: Medium amber coloured clear solution without any precipitate.
Reaction: Reaction of 3.26% w/v aqueous solution at 25°C. pH: 7.0±0.2
pH
6.80-7.20
Cultural Response
Cultural characteristics observed after an incubation at 35-38°C for 18-24 hours.
| Organism | Growth | Esculin hydrolysis |
|---|---|---|
| Enterococcus bovis (27960) | luxuriant | + |
| Enterococcus faecium ATCC 19434 (00010*) | luxuriant | + |
| Escherichia coli ATCC 25922 (00013*) | inhibited | - |
Key: (*) Corresponding WDCM numbers.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (1,2).
| Product Name | Kanamycin Esculin Azide Broth Base |
|---|---|
| SKU | M776A |
| Customized Product Available | No |
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