Ashby’s Glucose Agar

Ashby’s Glucose Agar is used for cultivation of Azotobacter species from soil that can use glucose and atmospheric nitrogen as source of carbon and nitrogen respectively. Azotobacters are a non-symbiotic nitrogen fixing bacteria and they also synthesize biologically active substances which attributes to improving seed germination, plant growth etc. Dipotassium phosphate provides buffering to the system. Various essential ions required for promoting growth of Azotobacter are also available in this medium.