Casitone Glycerol Yeast Autolysate Broth Base (CGY)

Casitone Glycerol Yeast Autolysate Broth Base (CGY) is recommended for maintenance of iron bacteria especially those belonging to the Sphaerotilus-Leptothrix group as per APHA. Pure cultures are isolated from BOD-Lactate Broth by picking a filament and streaking on 0.05% Meat Extract Agar. BOD- Lactate Broth acts as a partially selective medium for Sphaerotilus . After 24 hours incubation at 25°C, the typical curling filaments are transferred to CGY Broth, which is a good maintenance medium. If a pellicle with no underlying turbidity develops in 2 to 3 days, filament is transferred to CGY Agar slant.