Disinfectant Test Broth, AOAC medium is used for daily transfer of cultures required in testing disinfectants and especially for determining phenol coefficients of disinfectants. The medium contains the ingredients like HM peptone B and Peptone, which provide necessary nutrients tothe growth of test organisms like Pseudomonas aeruginosa and Staphylococcus aureus. Presence of sodium chloride balances the osmotic equilibrium.