This medium is prepared according to the formulation by Demerec for cultivation of Drosophila. For the culture of Drosophila, molten medium is poured into bottles or flasks to a depth of about 2 inches. After the medium is poured into bottles, strips of filter paper cut to the length of bottle are inserted to provide a place for the flies to pupate. The surface of the medium is then inoculated with heavy suspension of live yeast to induce the adult flies to lay eggs. A male and female fly possessing the desired genetic characters are then added to the bottles for mating. The cultures are incubated for about 12 days during which the flies begin to emerge.
1. Grove and Randall, 1955, Assay Methods of Antibiotics Medical Encyclopedia, Inc, New York. 2.Schmidt and Moyer, 1944; J. Bact, 47:199. 3.Tests and Methods of Assay of Antibiotics and Antibiotic containing Drugs, FDA, CFR, 198 3.Title 21, part 436, SubpartD, Washington, D.C. U.S Government printing office, paragraphs 436, 100-436, 106 pg 242-259 (April 1).