Nutrient Agar w/ tryphan blue

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SKU:
M2051
For detection and enumeration of aerobic endospore formers from water samples by membrane filtration.


Nutrient Agar w/Trypan blue is recommended by APHA for detection and enumeration of aerobic endospore formers from water samples by membrane filtration. The water samples under test is exposed to heat treatment wherein vegetative cells are destroyed and aerobic spores remain unaffected. The water sample is then filtered through membrane filter and placed on Agar plate. Incubate at 35±0.5°C for 24 ± 2 hours. Further incubation up to 5-7 days for intracellular formation of endospores. Trypan blue in the medium imparts a coloured background to the membrane. The aerobic endospores hence can be easily visualized and enumerated..
More Information
Product Name Nutrient Agar w/ tryphan blue
SKU M2051
Product Type Regular
Physical Form Powder
Origin Animal
Packaging type HDPE
References 1. American Public Health Association, Standard Methods for the Examination of Dairy Products, 1978, 14th Ed., WashingtonD.C.2.Salfinger Y., and Tortorello M.L. Fifth (Ed.), 2015, Compendium of Methods for the Microbiological Examination of Foods,5th Ed., American Public Health Association, Washington, D.C.3.Baird R.B., Eaton A.D., and Rice E.W., (Eds.), 2015, Standard Methods for the Examination of Water andWastewater, 23rd ed., APHA, Washington, D.C.4.Isenberg, H.D. Clinical Microbiology Procedures Handbook. 2nd Edition.5.Jorgensen,J.H., Pfaller , M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015)Manual of Clinical Microbiology, 11th Edition. Vol. 1.6.Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17thEd., APHA Inc., Washington, D.C.
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