Tryptone Yeast Sodium Sulphite Agar Base

Tryptone Yeast Sodium sulphite Agar Base is recommended for the enumeration of Clostridium perfringens from water samples using membrane filtration technique. The composition and performance criteria of this medium are as per the specifications laid down in ISO 14189:2013. Clostridium perfringens reduces sulphite and imparts blackening to the medium. Presence of C.perfringens is confirmed by acid phosphatase test. This is indicated by development of strong purplish colour within 3-4 minutes on addition of Acid phosphatase reagent, R096.