EC 0157:H7 Selective Broth is recommended for the isolation of Escherichia coli O157:H7 from food samples. The media provides Proteose peptone and yeast extract as carbon and nitrogen sources, long chain amino acids, vitamins and minerals. Phosphates buffer the medium. Magnesium sulphate and ferrous sulphate are sources of ions that simulate metabolism. Sodium chloride maintains the osmotic balance. Mannitol serves as a carbon source.