Diagnostic Thioglycollate Medium (Thioglycollate Medium w/o Indicator)

This medium is nutritious and favours the growth of Clostridium butyricum, Campylobacter species, Bacteroides species and Pneumococci etc. from minimal inocula. Brucella species which fail to grow in the presence of indicator, can grow in this medium. The broth with addition of v/v serum may be used for cultivation of Trichomonas vaginalis. It can also be used as transportation medium for which calcium carbonate is incorporated in the medium. Calcium carbonate neutralizes the acid produced during growth and avoid rapid growth and death of gram-negative cocci, Clostridium perfringens and other acid-sensitive bacteria.