King’s Medium B Base w/ 1.5% Agar

Kings Medium B Base w/ 1.5% Agar is recommended for the non-selective isolation, cultivation and pigment production of Pseudomonas species in accordance with FDA BAM. Pigments and/or their derivatives produced by Pseudomonas species play a role as siderophores in the iron uptake systems of bacteria, and hence, their production is markedly enhanced under conditions of iron deficiency. The production of pigments especially non-fluorescent blue pigment, pyocyanin is readily demonstrated by culturing on Kings Medium B Base w/ 1.5% Agar, which contains no added iron. The addition of dipotassium phosphate increases the phosphorus content of the medium thereby enhancing production of fluorescent pigment.