YPD Broth (YEPD Broth)

YPD Broth (YEPD Broth) is recommended for the growth of Saccharomyces cerevisiae for molecular biology purposes.

Composition**

**Formula adjusted, standardized to suit performance parameters

Directions

Suspend 50.0 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

Principle And Interpretation

YPD Broth (YEPD Broth) is recommended by Sherman (1) for the growth of Saccharomyces cerevisiae for molecular biology purposes. This medium supports the growth of most heterotrophic microorganisms but due to their simple composition, they have been adopted as the basal media for the routine cultivation of yeasts. General methods in yeast genetics specify using Yeast Extract Peptone- Dextrose (YPD) medium for cultivating

S. cerevisiae and other yeasts (2). Yeasts grow well on a minimal medium containing only dextrose and salts. The addition of protein and yeast cell extract hydrolysates allows faster growth so that during exponential or log-phase growth, the cells divide every 90 minutes (2).

The medium composition aids in growth of Saccharomyces. Peptic digest of animal tissue provides nitrogenous nutrients. Yeast extract provides nitrogenous nutrients as well as Vitamin B Complex. Dextrose provides the carbohydrate and energy source to support growth of S. cerevisiae

Quality Control

Appearance
Cream to yellow homogeneous free flowing powder

Colour and Clarity of prepared medium
Light amber coloured clear solution in tubes.

Reaction
Reaction of 5.0% w/v aqueous solution at 25°C. pH: 6.5±0.2

pH
6.30-6.70

Cultural Response
M1363: Cultural characteristics observed after an incubation at 25-30°C for 48-72 hours.

*Key: Formerly known as Aspergillus niger

Storage and Shelf Life

Store below 30°C in tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label.

Reference

1. Sherman F., Meths. Enzymol. 194, 3 (1991).
2. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A. and Struhl K., 1994, Current protocols in molecular biology, Current Protocols, Brooklyn, N.Y.

Disclaimer

User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained in this and other related HiMedia™ publications. The information contained in this publication is based on our research and development work and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes to specifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use but for laboratory, diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not be considered as a warranty of any kind, expressed or implied, and no liability is accepted for infringement of any patents.