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Synthetic Sea Salt
Synthetic Sea Salt is recommended for preparation of special diluents.
Composition**
| Ingredients | Gms/Litre |
|---|---|
| Calcium chloride, 2H2O | 0.836 |
| Potassium chloride | 0.435 |
| Strontium chloride, 6H2O | 0.0007 |
| Sodium bicarbonate | 0.1515 |
| Magnesium sulphate, 7H2O | 3.800 |
| Magnesium chloride, 6H2O | 2.940 |
| Borax | 0.030 |
| Sodium chloride | 14.900 |
| Final pH (at 25°C) | 7.5±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 19.38 grams (the equivalent weight of dehydrated medium per litre) in 1000ml distilled water.Heat, if necessary, to dissolve the medium completely.Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well and dispense as desired.
Principle And Interpretation
The coliform group consists of several genera of bacteria belonging to the family Enterobacteriaceae (1). Coliforms can be detected by performing the multiple tube test so as to obtain the most probable number (MPN). Synthetic Sea Salt, recommended by ISO Committee (2) is used to prepare special diluents. It is used as a diluent during detection and enumeration of Escherichia coli and coliforms in surface and wastewater. Sodium chloride in the medium helps to maintain osmotic balance and the other ingredients provide buffering action.
Quality Control
Appearance White to cream homogeneous free flowing powder
Colour and Clarity of prepared medium Colourless clear solution without any precipitate
Reaction Reaction of 1.93% w/v aqueous solution at 25°C. pH: 7.5±0.2
pH 7.30-7.70
Cultural Response M1344: Satisfactory results are obtained when used as a diluent during detection and enumeration of E.coli and coliform bacteria in surface and wastewater.
Storage and Shelf Life
Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.
Reference
- Eaton A. D., Clesceri L. S. and Greenberg A. E., (Ed.), 1998, Standard Methods for the Examination of water and Wastewater, 20th Ed., American Public Health Association, Washington, D.C.
- International Organization for Standardization (ISO), 1995, Draft ISO/DIS 9308-3: 1998 (E) Pg No. 14.
| Product Name | Synthetic Sea Salt |
|---|---|
| SKU | M1344 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Chemically defined |
| Packaging type | HDPE |
| References | 1. Eaton A. D., Clesceri L. S. and Greenberg A. E., (Ed.), 1998, Standard Methods for the Examination of water and Wastewater,20th Ed., American Public Health Association, Washington, D.C. |
| Customized Product Available | No |









