Listeria Motility Medium is recommended for testing motility of Listeria monocytogenes. This media is formulated in accordance with ISO Committee specification for the determination of motility by L. monocytogenes. The motility of L. monocytogenes is best demonstrated by stab inoculating two tubes of semisolid medium and incubating one at room.
temperature (20 - 25°C) and the other at 35°C. Listeria
monocytogenes requires room temperature incubation before motility develops, since in some organisms; flagellar proteins develop more rapidly at lower temperatures (room temperature) such as in L. monocytogenes and Yersinia enterocolitica.
1. International Organization for Standardization (ISO), 1993, Draft ISO/DIS 10560.2.Bailey and Scotts Diagnostic Microbiology, 1986, 7th Ed., The C.V. Mosby Co., St. Louis.